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Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions |
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| Authors: | Vaclav Diopan Violetta Shestivska Ondrej Zitka Michaela Galiova Vojtech Adam Jozef Kaiser Ales Horna Karel Novotny Miroslav Liska Ladislav Havel Josef Zehnalek Rene Kizek |
| Affiliation: | 1. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ‐613 00 Brno, Czech Republic;2. Department of Plant Biology, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ‐613 00 Brno, Czech Republic;3. Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ‐611 37 Brno, Czech Republic;4. Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ‐613 00 Brno, Czech Republic;5. Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, Technicka 2896/2, CZ‐616 69 Brno, Czech Republic;6. University Institute, Tomas Bata University, T.?G. Masaryka 275, CZ‐762 72 Zlin, Czech Republic;7. RADANAL Ltd, Okruzni 613, CZ‐53003 Pardubice, Czech Republic |
| Abstract: | The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC‐ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC‐CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy. |
| Keywords: | Thiols Liquid chromatography Coulometric detection Amperometric detection Heavy metal Laser induced breakdown spectroscopy |