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Activity and Enantioselectivity of the Hydroxynitrile Lyase MeHNL in Dry Organic Solvents |
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| Authors: | Monica Paravidino Dr Menno J Sorgedrager Dr Romano V A Orru Prof Dr Ulf Hanefeld Dr |
| Institution: | 1. Gebouw voor Scheikunde, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft (The Netherlands), Fax: (+31)?15 278 1415;2. CLEA Technologies B.V. Delftechpark 34, 2628 XH Delft (The Netherlands);3. Department of Chemistry and Pharmaceutical Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam (The Netherlands), Fax: (+31)?20 598 74 88 |
| Abstract: | Water concentration affects both the enantioselectivity and activity of enzymes in dry organic media. Its influence has been investigated using the hydrocyanation of benzaldehyde catalyzed by hydroxynitrile lyase cross‐linked enzyme aggregate (MeHNL‐CLEA) as a model reaction. The enzyme displayed higher enantioselectivity at higher water concentration, thus suggesting a positive effect of enzyme flexibility on selectivity. The activity increased on reducing the solvent water content, but drastic dehydration of the enzyme resulted in a reversible loss of activity. |
| Keywords: | enzyme catalysis hydroxynitrile lyase immobilization organic solvents reaction medium water |