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The Development of Highly Sensitive and Selective Immunosensor Based on Antibody Immobilized ZnO Nanorods for the Detection of D‐Dimer |
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| Authors: | Zafar H. Ibupoto Nikolaos Mitrou Georgia‐Paraskevi Nikoleli Dimitrios P. Nikolelis Magnus Willander Nikolas Psaroudakis |
| Affiliation: | 1. Physical Electronic and Nanotechnology Division, Department of Science and Technology, Campus Norrk?ping, Link?ping University, SE‐60174 Norrk?ping, Sweden;2. Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis‐Kouponia, GR‐15771 Athens, Greece tel: ++302107274754;3. Laboratory of Inorganic & Analytical Chemistry, School of Chemical Engineering, Dept 1, Chemical Sciences, National Technical University of Athens, 9 Iroon Polytechniou St., Athens 157 80, Greece;4. Laboratory of Inorganic Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis‐Kouponia, GR‐15771 Athens, Greece |
| Abstract: | ZnO nanorods were grown on gold coated glass substrate by low temperature aqueous chemical growth method. Scanning electron microscopy (SEM) and X‐ray diffraction (XRD) techniques were used for the characterization of ZnO nanorods. ZnO nanorods are highly dense, uniform, well aligned and perpendicular‐oriented to the substrate. ZnO nanorods exhibited good crystal quality. The well aligned ZnO nanorods were potentially used for the development of selective and sensitive immunosensor for the detection of D‐dimer by immobilizing antibody on stabilized lipid films. The ZnO nanorods based immunosensor responded to a wide range of D‐dimer concentrations with fast response time of ca. 20 s. |
| Keywords: | ZnO nanorods D‐Dimer, Immunosensors Potentiometric technique |