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Construction of an Amperometric Glucose Biosensor by Immobilization of Glucose Oxidase on Nanocomposite at the Surface of FTO Electrode |
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| Authors: | Zahra Rezay Marand Nasser Shahtahmassebi Mohammad Reza Housaindokht Golam Hossien Rounaghi Iman Razavipanah |
| Institution: | 1. Departmentof Physic, Ferdowsi University of Mashhad, Mashhad, Iran;2. Nanoresearch Center, Ferdowsi University of Mashhad, Mashhad, Iran;3. Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, Iran tel: 0098‐511‐7626388;4. fax: 0098‐511‐8796416 |
| Abstract: | Fluorine? tin oxide (FTO) nanostructure was developed on the surface of a glass plate using spray payroliziz method. A new electrochemical biosensor was fabricated based on a layer by layer process. In this process chitosan? Fe3O4 (CH? Fe3O4) nanocomposite film was prepared at the surface of FTO electrode by dip? coating method. In the next step, the glucose oxidase (GOx) was immobilized on the CH? Fe3O4/FTO nanocomposite electrode. The GOx/CH? Fe3O4/FTO bioelectrode has a linear range of 10–270 µM and a detection limit of 5 µM. The highest sensitivity was obtained at 1.2 µA mM?1 cm?2. |
| Keywords: | Glucose biosensors FTO electrodes Fe3O4 nanoparticles Chitosan Glucose oxidase |