Simultaneous Detection of Homocysteine and Cysteine in the Presence of Ascorbic Acid and Glutathione Using a Nanocarbon Modified Electrode |
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| Authors: | P T Lee D Lowinsohn R G Compton |
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| Institution: | 1. Department of Chemistry, Physical and Theoretical Chemistry Laboratory, Oxford University, South Parks Road, Oxford, OX1?3QZ, UK;2. Department of Chemistry, Instituto de Ciências Exatas, Universidade Federal de Juiz de Fora, 36036‐330, Juiz de Fora, MG, Brazil. |
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| Abstract: | The simultaneous electrochemical detection of homocysteine and cysteine using an absorbed ortho‐quinone species, catechol, at the nanocarbon modified glassy carbon electrode was achieved via 1,4‐Michael addition reaction. The detection was done in the presence and the absence of each other as well as with both glutathione and ascorbic acid present in order to investigate the selectivity of homocysteine and cysteine. A determination of homocysteine sensitivity is (0.882±0.296) nA nM?1 with a LOD of ca. 11 nM and cysteine sensitivity is (7.501±0.202) mA µM?1 with a LOD of ca. 5.0 µM within a range of 0–0.1 mM. |
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| Keywords: | Catechol ortho‐Quinone Nanocarbon Carbon electrodes 1 4‐Michael addition reaction Thiols Cysteine Homocysteine |
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