A Highly Sensitive and Selective Enzymatic Biosensor Based on Direct Electrochemistry of Hemoglobin at Zinc Oxide Nanoparticles Modified Activated Screen Printed Carbon Electrode |
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| Authors: | Selvakumar Palanisamy Chelladurai Karuppiah Shen‐Ming Chen Prakash Periakaruppan |
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| Institution: | 1. Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.?1, Section?3, Chung‐Hsiao East Road, Taipei 106, Taiwan;2. Post Graduate and Research Department of Chemistry, Thiagarajar College, Madurai‐625009, Tamilnadu, India fax: +886?2270?25238;3. tel: +886?2270?17147 |
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| Abstract: | A sensitive enzymatic biosensor has been developed for the detection of hydrogen peroxide (H2O2), nitrite ( ) and trichloroacetic acid (TCA) by using hemoglobin (Hb) immobilized on activated screen printed carbon electrode (ASPCE) and zinc oxide (ZnO) composite. A pair of well defined redox peaks is observed with a heterogeneous electron transfer rate constant (Ks) of 5.27 s?1 for Hb at ASPCE/ZnO. The biosensor exhibits the detection of H2O2, TCA and  in the concentration range of 0.5–129.5 µmol L?1, 2.5–72.5 mmol L?1 and 0.2–674 µmol L?1 with the detection limit of 0.083 µmol L?1, 0.12 mmol L?1 and 0.069 µmol L?1, respectively. |
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| Keywords: | Activated screen printed carbon electrode Zinc oxide Hemoglobin Direct electrochemistry Biosensors |
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