Development of an Amperometric Microbial Biosensor Based on Thiobacillus thioparus Cells for Sulfide and Its Application to Detection of Sulfate‐Reducing Bacteria |
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Authors: | Peng Qi Dun Zhang Yi Wan |
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Affiliation: | 1. Key Laboratory of Marine Environmental Corrosion and Bio‐fouling, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China;2. University of the Chinese Academy of Sciences, 19 (Jia) Yuquan Road, Beijing 100039, China |
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Abstract: | This paper describes a novel electrochemical microbial biosensor based on Thiobacillus thioparus cells for sulfide detection. The morphology and electrochemical properties of the proposed biosensor were characterized by SEM and cyclic voltammetry, respectively. Working conditions of the microbial biosensor were optimized to obtain good electrochemical performances. Under the optimum conditions, analytical performances were evaluated, and the results suggested that the microbial biosensor could be used for selective detection of sulfide. The microbial biosensor was then successfully applied in detection of sulfate‐reducing bacteria by oxidizing its characteristic metabolite, sulfide, which was accumulated in culture media during bacterial growth. |
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Keywords: | Electrochemical Biosensors Sulfate‐reducing bacteria Sulfide |
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