Concerted Determination of the Hydrogen Atom and Electron Transfer Capacity of Lipid Soluble Reducing Agents |
| |
Authors: | Solomon Mengistu Lemma Matteo Scampicchio Andrea Bulbarello Marco Mason Loni Schweikert |
| |
Institution: | 1. Free University of Bolzano, Piazza Università 5, 39100 Bolzano, Italy;2. DSM Nutritional Products Ltd. Nutrition Innovation Center Research and Development Forms and Application, CH‐4002 Basel, Switzerland |
| |
Abstract: | This paper describes a method based on square wave voltammetry to evaluate either the electron transfer or the hydrogen atom transfer of lipid soluble antioxidants such as dl ‐mix‐tocopherol, BHT, ethoxyquin and retynil acetate. The electron transfer (ET) capacity was evaluated by the peak current, peak potential and the area under the anodic wave, whereas the hydrogen atom transfer (HAT) capacity by the kinetic rate of the reaction between antioxidants and 2,2‐Azobis(2‐methylpropionamidine) dihydrochloride (AAPH). The results indicate that ethoxyquin and tocopherol have the highest ET and HAT capacity. However, HAT capacity of tocopherol, BHT and retinyl acetate depend on the concentration. The approach has the advantage to assess HAT and ET capacity of lipid soluble antioxidant in a single concerted protocol. |
| |
Keywords: | Antioxidant AAPH Hydrogen atom transfer Electron transfer Square wave voltammetry |
|
|