Chitosan/AuNPs Modified Graphene Electrochemical Sensor for Label‐Free Human Chorionic Gonadotropin Detection |
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| Authors: | Sofia Teixeira Nadia S Ferreira Robert Steven Conlan O J Guy M Goreti F Sales |
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| Institution: | 1. College of Engineering, Swansea University, Singleton Park, Swansea, UK;2. College of Medicine, Swansea University, Singleton Park, Swansea, UK;3. BioMark/ISEP, School of Engineering, Polytechnic Institute of Porto, Porto, Portugal;4. BioMark, Sensor Research, Instituto Superior de Engenharia do Porto, R. Dr. António Bernardino de Almeida, 431, 4200‐072 Porto, Portugal tel: +351228340544;5. fax: +351228321159 |
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| Abstract: | A new immunosensor is presented for human chorionic gonadotropin (hCG), made by electrodepositing chitosan/gold‐nanoparticles over graphene screen‐printed electrode (SPE). The antibody was covalently bound to CS via its Fc‐terminal. The assembly was controlled by electrochemical Impedance Spectroscopy (EIS) and followed by Fourier Transformed Infrared (FTIR). The hCG‐immunosensor displayed linear response against the logarithm‐hCG concentration for 0.1–25 ng/mL with limit of detection of 0.016 ng/mL. High selectivity was observed in blank urine and successful detection of hCG was also achieved in spiked samples of real urine from pregnant woman. The immunosensor showed good detection capability, simplicity of fabrication, low‐cost, high sensitivity and selectivity. |
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| Keywords: | Immunosensor Screen‐printed electrode Graphene Antibody Chitosan Human chorionic gonadotropin |
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