Composite Material Based on Macroporous Polyaniline and Osmium Redox Complex for Biosensor Development |
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| Authors: | Riccarda Antiochia Franco Mazzei Lo Gorton Dónal Leech Gabriele Favero |
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| Institution: | 1. Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy;2. Department of Analytical Chemistry/Biochemistry and Structural Biology, Lund University, P.?O.Box 124, SE‐221?00 Lund, Sweden;3. School of Chemistry, National University of Ireland Galway, University Road, Galway, Ireland |
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| Abstract: | Here the feasibility of layers based on the conducting polymer polyaniline (PANI) as component of glucose biosensors using glucose oxidase (GOx) as enzyme and Os(bpy)2(4‐aminomethylpyridine)Cl]PF6 (OsCmplx) as electrochemical mediator, is evaluated. Particularly, PANI was employed to obtain a nanostructured macroporous material (m‐PANI) around polystyrene nanoparticles taken as template and the mediator was co‐immobilized during the polymerizing procedure. The GOx biosensor based on OsCmplx modified m‐PANI provides a linear response to glucose concentration in the range 5 up to 65 mM with a sensitivity of 3.54 µA/mM/cm2 (on a projected geometric area=0.07 cm2), an LOD of 0.8 mM and a good precision (%RSD≤7, n=5); the biosensor is stable showing a decrease of 10% to the value of the sensitivity after 15 days of use and of about 50% after 40 days. |
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| Keywords: | Polyaniline Macroporous material Osmium redox complex Glucose biosensor |
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