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Commercial Screen‐Printed Gold Electrodes for the Detection and Quantification of Aminothiols in Human Plasma by Liquid Chromatography with Electrochemical Detection
Authors:Santiago Cavanillas  Núria Serrano  José Manuel Díaz‐Cruz  Cristina Ariño  Miquel Esteban
Affiliation:Departament de Química Analítica, Universitat de Barcelona, Martí i Franquès, 1‐11, E‐08028 – Barcelona (Spain) phone: (+34) 93?403?91 16, fax: (+34) 93?402?12?33
Abstract:In order to show the possibilities of screen‐printed electrodes in HPLC detection, a method is presented for routine quantification of aminothiols in human plasma. It uses commercial gold screen‐printed electrodes and provides a rapid, simple and economical procedure with a disposable detector and without any additional electrode. By working at 98 : 2 (0.05 % trifluoroacetic acid: methanol) and 0.9 V, it allows us to determine cysteine (Cys), cysteinyl‐glycine (Cys‐Gly), and homocysteine (HCys) at μmol/L levels. The method was validated, and linearity range, detection and quantification limits, precision and accuracy were evaluated by external calibration and by using N‐acetylcysteine as internal standard, which exhibited better precision.
Keywords:Liquid chromatography  Electrochemical detection  Gold screen‐printed electrode  Aminothiols  Human plasma
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