Determination by the enzyme thermistor of cellobiose formed on degradation of cellulose |
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| Authors: | B. Danielsson E. Rieke B. Mattiasson F. Winquist K. Mosbach |
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| Affiliation: | 1. Pure and Applied Biochemistry, Chemical Center, University of Lund, PO Box 740, S-220 07, Lund 7, Sweden
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| Abstract: | A calorimetric assay procedure for the determination of cellobiose has been developed. The cellobiose is hydrolyzed by β-glucosidase and the glucose formed is measured calorimetrically by an enzyme thermistor containing co-immobilized glucose oxidase and catalase. The system was optimized with regard to the arrangement of the enzymes, the pH-dependence of the separate enzymic steps, and of the total system. By placing the β-glucosidase in a precolumn that could be switched in and out of the flow through the enzyme thermistor, both cellobiose and glucose present in the sample could be determined. The performance with standard solutions and with crude samples from cellulose degradation experiments was investigated. |
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| Keywords: | Calorimetric assay, of cellobiose assay, calorimetric, of cellobiose enzyme thermistor, for cellobiose cellobiose, enzyme thermistor for thermistor, enzyme, for cellobiose immobilized glucose oxidase glucose oxidase, immobilized oxidase immobilized glucose catalase, immobilized immobilized catalase beta-glucosidase cellulose, determination of cellobiose formed on degradation of |
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