|
|||||
|
|
Automated Deconvolution of Overlapped Ion Mobility Profiles |
|
| Authors: | Matthew Brantley Behrooz Zekavat Brett Harper Rachel Mason Touradj Solouki |
| Affiliation: | 1. Department of Chemistry and Biochemistry, University of Texas at Tyler, Tyler, TX, 75799, USA 2. Department of Chemistry and Biochemistry, Baylor University, Waco, TX, 76706, USA 3. Institute of Biomedical Studies, Baylor University, Waco, TX, 76706, USA |
| Abstract: | Presence of unresolved ion mobility (IM) profiles limits the efficient utilization of IM mass spectrometry (IM-MS) systems for isomer differentiation. Here, we introduce an automated ion mobility deconvolution (AIMD) computer software for streamlined deconvolution of overlapped IM-MS profiles. AIMD is based on a previously reported post-IM/collision-induced dissociation (CID) deconvolution approach [J. Am. Soc. Mass Spectrom. 23, 1873 (2012)] and, unlike the previously reported manual approach, it does not require resampling of post-IM/CID data. A novel data preprocessing approach is utilized to improve the accuracy and efficiency of the deconvolution process. Results from AIMD analysis of overlapped IM profiles of data from (1) Waters Synapt G1 for a binary mixture of isomeric peptides (amino acid sequences: GRGDS and SDGRG) and (2) Waters Synapt G2-S for a binary mixture of isomeric trisaccharides (raffinose and isomaltotriose) are presented. Graphical Abstract ? |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |