| 胆固醇-7,8-脱氢酶大肠杆菌工程菌的构建 |
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| 引用本文： | 马志军,薛家禄,薛雯月,薛智文,刘向阳.胆固醇-7,8-脱氢酶大肠杆菌工程菌的构建[J].河南科学,2014(3):362-364. |
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| 作者姓名： | 马志军 薛家禄 薛雯月 薛智文 刘向阳 |
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| 作者单位： | 河南利伟生物药业股份有限公司,河南温县454850 |
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| 基金项目： | 国家高技术研究发展计划（863计划）课题（2011AA02A211） |
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| 摘 要： | 7-去氢胆固醇在化妆品和医药领域具有较大的应用需求,但现有的生产方法为化工法,流程复杂,且易污染环境.发酵法生产7-去氢胆固醇是一条新颖且具有潜在竞争力的生产途径.以虾Y-器官微粒体的cDNA文库为模板进行胆固醇-7,8-脱氢酶基因的扩增,与质粒载体连接后转化大肠杆菌,并对该工程菌的生物催化胆固醇-7,8-脱氢能力进行检测.
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| 关 键 词： | cDNA文库 重组大肠杆菌 胆固醇- -脱氢酶 |
Construction of Engineered Escherichia coli for Cholesterol-7, 8-dehydrogenase Production |
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| Ma Zhijun,Xue Jalu,Xue Wenyue,Xue Zhiwen,Liu Xiangyang.Construction of Engineered Escherichia coli for Cholesterol-7, 8-dehydrogenase Production[J].Henan Science,2014(3):362-364. |
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| Authors: | Ma Zhijun Xue Jalu Xue Wenyue Xue Zhiwen Liu Xiangyang |
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| Institution: | (Henan Liwei Biological Pharmaceutical Co. Ltd., Wenxian 454850, Henan China) |
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| Abstract: | 7-dehydrocholesterol has great application in cosmetic and pharmaceutical field,but the existing production method is chemical method, which is complex and environmentally harmfully. Steroid biotransformation has been a novel and competitive way. In this experiment,the cholesterol-7, 8-dehydrogenase gene was amplified by PCR from shrimp Y-organ microsome cDNA library, then it was cloned into plasmid, and the recombinant plasmid was transformed into E. coli. The transformation ability of cholesterol-7,8-dehydrogenase in the reconstructed strain was detected by fermentation. |
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| Keywords: | cDNA library： recombinant E coli cholesterol-7 8-dehydrogenase |
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