| 基于固相抗体模式时间分辨荧光免疫分析测定雌三醇 |
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| 引用本文： | 高素芹,杜凌云,王术皓.基于固相抗体模式时间分辨荧光免疫分析测定雌三醇[J].光谱实验室,2010,27(3):1089-1093. |
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| 作者姓名： | 高素芹 杜凌云 王术皓 |
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| 作者单位： | 聊城大学化学化工学院,山东省聊城市湖南路1号,252059 |
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| 基金项目： | 国家自然科学基金，山东省自然科学基金，山东省"泰山学者"建设工程专项经费资助 |
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| 摘 要： | 采用二步标记法,借助二乙烯三胺五乙酸酐,将Eu3+标记到雌三醇完全抗原上,基于固相抗体竞争反应模式,建立了时间分辨荧光免疫分析测定雌三醇的新方法。在优化的条件下,方法的线性范围为0.005—1000ng·mL-1,检出限为4pg·mL-1。该方法用于检测血清中雌三醇的含量,结果令人满意。
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| 关 键 词： | 固相抗体包被 时间分辨荧光免疫分析 雌三醇 铕 |
Time-Resolved Fluoroimmunoassay for Estriol Based on Solid Phase Coated Antibody Competitive Mode |
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| GAO Su-Qin,DU Ling-Yun,WANG Shu-Hao.Time-Resolved Fluoroimmunoassay for Estriol Based on Solid Phase Coated Antibody Competitive Mode[J].Chinese Journal of Spectroscopy Laboratory,2010,27(3):1089-1093. |
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| Authors: | GAO Su-Qin DU Ling-Yun WANG Shu-Hao |
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| Institution: | GAO Su-Qin DU Ling-Yun WANG Shu-Hao(College of Chemistry and Chemical Engineering,Liaocheng University,Liaocheng,Shandong 252059,P.R.China) |
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| Abstract: | A rapid and sensitive method based on solid phase coated antibody by time-resolved fluoroimmunoassay for the determination of estriol was established.The estriol-ovalbumin （E3-OVA） was labeled with europium ion （Eu3＋） by means of a simple method involving the anhydride of diethylenetriam inepentaacetia acia（DTPAA）.Under the optimal conditions,the linear range of the method was from 0.005 to 1000ng·mL-1,and the limit of detection was found to be 4pg·mL-1.The method has been applied to determine estriol in serum sample with satisfactory results. |
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| Keywords: | Solid Phase Coated Antibody Time-Resolved Fluoroimmunoassay Estriol Europium |
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