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Expression and Immunogenicity of a Recombinant Chimeric Protein of Human Colorectal Cancer Antigen GA733-2 and an Fc Antibody Fragment in Stably Transformed Drosophila melanogaster S2 Cells |
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| Authors: | Yeon Ju Seok Kyung Il Kim Ki Hyun Yoo Jeon Hwang-Bo Hyun Ho Lee Dong Hwa Shon Ki Sung Ko Hyung Sik Kang Kyung Jin Lee Doo-Byoung Oh Young Hee Joung In Sik Chung |
| Institution: | 5. Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Suwon, 446-701, Korea 1. Food Immunology Research Group, Korea Food Research Institute, Seongnam-si, Kyunggi-do, 463-746, Korea 2. Department of Life Science, WonKwang University, Iksan, 156-756, Korea 3. School of Biological Science and Technology, Chonnam National University, Gwangju, 500-757, Korea 4. Integratve Omics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yuseong-gu, Daejeon, 305-806, Korea |
| Abstract: | Human colorectal cancer antigen GA733-2 fused to the immunoglobin Fc fragment (GA733-2-Fc) was expressed in stably transformed Drosophila melanogaster S2 cells, and the immunogenicity of recombinant GA733-2-Fc was investigated in mice. Recombinant GA733-2-Fc was secreted into a culture medium with a molecular mass of approximately 65 kDa. Recombinant GA733-2-Fc was purified to homogeneity using affinity fractionation with Protein A sepharose 4 Fast Flow. Recombinant GA733-2-Fc proteins elicited production of specific antibodies against recombinant GA733-2 by immunization through an intraperitoneal route. Recombinant GA733-2-Fc-induced antibodies showed a binding activity to human colorectal carcinoma HCT-116 cells. Secretory recombinant GA733-2-Fc from Drosophila S2 cell systems can be used as an effective experimental antigen for research in cancer vaccine development. |
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