Efficiency of porous hydrophilic polymer-based packing materials in chromatographic analysis of drugs existing with polypeptide

Summary A size monodispersed restricted-access polymeric packing material has been prepared through a simple and easy one-step method of co-polymerization of glycerol monomethacrylate and glycerol dimethacrylate with cyclohexanol as prorogen. A typical seeded polymerization (two step swelling and polymerization method) in an aqueous medium gave a 90 % yield of porous beads which could be utilized as a packing material in high performance liquid chromatography (HPLC) without any size classification due to the excellent size monodispersity. A BET measurement and size exclusion chromatography in either tetrahydrofuran or water revealed that the prepared material had only small pores (around 100 Å in diameter). It showed ample hydrophobicity for the separation of hydrophilic drug molecules in 10% aqueous acetonitrile buffer, while polypeptides such as bovine serum albumin were excluded and eluted before the void volume of the column with quantitative recovery.