过氧化物模拟酶催化的苯基荧光酮氧化反应及其分析应用

在ＮＨ４Ｃｌ－ＮＨ４ＯＨ缓冲介质中，氯化血红素（Ｈｅｍｉｎ）有显著的过氧化物模拟酶活性，催化过程化氢氧化苯基荧光酮褪色。本文探讨了反应机理，比较Ｈｅｍｉｎ与天然酶催化性能，考察反应条件和共存物质影响，从而提出测定Ｈｅｍｉｎ和过氧化氢的高灵敏分光光度法，线性范围分别为０￣３．０×１０＾－８ｍｏｌ／Ｌ和０￣１．２×１０＾５ｍｏｌ／Ｌ；检测限（３σ）分别为１．８×１０＾－１０ｍｏｌ／Ｌ和１．４×１０＾７

Mimetic Peroxidase-Catalyzed Oxidation of Phenylfluorone and Its Analytical Applications

In ammonium buffer medium, hemin exhibits efficient peroxidase - like catalytic activity on the oxidation of phenylfluorone by hydrogen peroxide. The reaction mechanism, catalytic property of hemin, optimum reaction conditions and effects of coexisting substances were investigated. Based on the catalytic effect, procedures were presented for highly sensitive spectrophotometric determination of hemin and hydrogen peroxide, with limits of detection of 1. 8 x 10-10 mol/L and 1. 4 x 10-7 mol/L, respectively. By coupling with a glucose oxidase-catalyzed reaction, glucose in human serum was determined with satisfactory results.