Isolation and determination of ginsenosides in American ginseng leaves and root extracts by LC-MS |
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Authors: | T Ligor A Ludwiczuk T Wolski B Buszewski |
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Institution: | (1) Department of Environmental Chemistry and Ecoanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Street, 87100 Toruń, Poland;(2) Department of Pharmacognosy with Medicinal Plant Laboratory, Skubiszewski Medical University, 1 Chodźki Street, 20093 Lublin, Poland |
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Abstract: | Ginseng saponins (ginsenosides) were extracted from the root and leaves of locally cultivated American ginseng (Panax quinquefolium L.). For the isolation of compounds from plant samples three different extraction methods were utilized: accelerated solvent
extraction, the ultrasound-assisted solvent extraction and mechanical shaking assisted solvent extraction. The separation
of compounds was achieved with a water–acetonitrile gradient system using a C18 reversed-phase column. Target compounds were
identified in MS2 and MS3 experiments. The relative distribution of these ginsenosides in each root and leaf extract was established. The limit of
detection of the method was less than 30 ng/ml. Recovery of ginseng saponins in spiked samples exceeded 80%, while the relative
standard deviation ranged from 7.1 to 9.1%. The total concentrations of ginsenosides were 41 and 13 mg/g in root and leaves. |
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Keywords: | American ginseng Leaves Extraction Liquid chromatography/mass spectrometry |
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