Analysis of non-covalent protein complexes by capillary electrophoresis--time-of-flight mass spectrometry |
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Authors: | Brenner-Weiss Gerald Kirschhöfer Frank Kühl Boris Nusser Michael Obst Ursula |
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Institution: | Forschungszentrum Karlsruhe, Institut für Technische Chemie, Bereich Wasser- und Geotechnologie Postfach 36 40, 76021 Karlsruhe, Germany. gerald.brenner-weiss@itc-wgt.fzk.de |
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Abstract: | A capillary electrophoresis-electrospray ionisation time-of-flight mass spectrometry (CE-ESI-TOF-MS) method for characterisation of non-covalent protein complexes is described using a coaxial liquid sheath-flow sprayer. The CE capillary was connected to the mass spectrometer using a commercial CE-MS sprayer mounted on a ceramic holder of the ESI interface of the mass spectrometer. Using myoglobin (Mb) as an example of non-covalent protein complex, the effect on complex stability caused by organic modifiers added to the sheath liquid was analysed. Depending on the amount of methanol, either intact Mb or the apoprotein and the prosthetic heme group were detected. |
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