Miniaturization of solid-phase reactors for on-line post-column derivatization in narrow-bore liquid chromatography |
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Authors: | H Jansen U A Th Brinkman R W Frei |
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Institution: | (1) Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands |
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Abstract: | Summary The use of solid-phase reactors for post-column derivatization in narrow-bore HPLC (1.0mm i.d. analytical columns) is evaluated.
Two systems are described, viz. for the determination of N-methylcarbamate pesticides and for that of urea and ammonia. The
solid-phase reactor is packed with a strong anion exchange resin and urease immobilized on silica, respectively, to effect
the catalytic hydrolysis of the solutes eluting from the analytical column. In both systems, the hydrolysis product is reacted
with o-phthalaldehyde followed by fluorescence monitoring.
Analytical data are presented and band broadening from various parts of the reaction detector system is discussed. An on-line
trace enrichment procedure via a micro precolumn is descried for the trace level determination of N-methylcaramates in surface
water samples. |
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Keywords: | Narrow-bore column liquid chromatography Post-column derivatization Solid-phase reactors Catalytic hydrolysis On-line trace enrichment Immobilized enzyme |
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