Development and validation of an HPLC/MS/MS method for the determination of sufentanil and morphine in human plasma |
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Authors: | Manfio Josélia Larger Santos Verônica Jorge Lanchote Vera Lúcia Santos Luciana M Carmona Maria José C Auler José Otavio Júnior Brum Liberato Júnior Donaduzzi Carmen Maria Santos Silvia R C J |
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Affiliation: | School of Pharmaceutical Sciences USP/SP, Av. Lineu Prestes, 580 Bloco 13a, Butant?-Cidade Universitária, CEP 05508-900, S?o Paulo-SP Brazil. manfiojoselia@bol.com.br |
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Abstract: | A sensitive and fast HPLC/MS/MS method for measurement of sufentanil and morphine in plasma was developed and validated. A single liquid-liquid extraction in alkaline medium was used for the cleanup of plasma, and fentanyl was added as an internal standard (IS). The analyses were carried out using a C18 column and the mobile phase acetonitrile-5 mM ammonium acetate + 0.25% formic acid (70 + 30, v/v). The triple-quadrupole mass spectrometer equipped with an electrospray source in positive mode was set up in the selective reaction monitoring mode to detect precursor --> product ion transition 387.0 > 238.0, 285.7 > 165.1, and 337.0 > 188.0 for sufentanil, morphine, and IS, respectively. The method was linear in the 0.05 (LOQ) - 500 ng/mL range for sufentanil and 10 (LOQ) - 1000 ng/mL range for morphine. Good selectivity, linearity, precision, accuracy, and robustness were obtained for the HPLC/MS/MS method. The proposed method was successfully applied for the determination of sufentanil and morphine in patients undergoing cardiac surgery. |
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