Spectral-luminescent parameters of the amniotic fluid |
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Authors: | E. I. Slobozhanina N. M. Kozlova L. P. Kas’ko M. V. Mamontova E. A. Chernitskii |
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Affiliation: | (1) Institute of Photobiology, National Academy of Sciences of Belarus, 27, F. Skorina Ave., 220072 Minsk, Belarus;(2) Belarusian Institute for Advanced Physician Training of the Ministry of Public Health of the Republic of Belarus, Minsk, Belarus |
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Abstract: | Absorption and luminescence excitation and emission spectra of the amniotic fluid from females with a normal course of pregnancy and with fetation anomalies are investigated. It is shown that the amniotic fluid manifests, in addition to an intense UV luminescence, a luminescence in the range of 380–650 nm. The first luminescence peak with λmax = 430–450 nm is most likely due to the presence of NADH2 and hormones in the amniotic fluid, whereas the peak located at longer wavelengths with λmax = 520–540 nm is due to pigments (bilirubin, etc.). Differences in absorption spectra of the amniotic fluid in the case of the normal course of pregnancy and upon its pathologies are established. Luminescence emission and excitation spectra of the amniotic fluid in cases of intrauterine fetation pathologies are shifted toward the longwave region compared to normal pregnancy cases. It is concluded that the spectral-luminescent analysis of the amniotic fluid can be used as an additional rapid test for monitoring of intrauterine fetal pathologies. Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 65, No. 6, pp. 939–942, November–December, 1998. |
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Keywords: | amniotic fluid absorption spectra luminescence risk of congenital fetal anomalies |
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