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Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems |
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| Authors: | Pedro P. Madeira, Jos A. Teixeira, Eug nia A. Macedo, Larissa M. Mikheeva,Boris Y. Zaslavsky |
| Affiliation: | aLSRE - Laboratory of Separation and Reaction Engineering, Dpt. de Engenharia Química, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal bIBB-Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga, Portugal cAnaliza Inc., 3615 Superior Ave., Suite 4407b, Cleveland, OH 44114, USA |
| Abstract: | Distribution coefficients for a variety of proteins and certain other biomolecules (peptides, amino acids, and carbohydrates) (overall 27 different solutes) were measured in aqueous two-phase systems (ATPSs) dextran (Dex)–polyethylene glycol (PEG) and Dex–Ucon 50-HB-5100 (Ucon—a random copolymer of ethylene glycol and propylene glycol) both containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4, at 23 °C. Distribution coefficients of some selected solutes were also measured in the above two-phase systems at three different polymer concentrations for each system. It was established that the distribution coefficients for all the proteins examined in the ATPSs are correlated according to the so-called Collander linear equation. |
| Keywords: | Aqueous two-phase systems Protein partitioning Collander equation |
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