An ultrasound assisted extraction of the available humic substance from marine sediments |
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Authors: | Mecozzi M Amici M Pietrantonio E Romanelli G |
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Affiliation: | Istituto Centrale per la Ricerca Scientifica e Tecnologica Applicata al Mare, Via di Casalotti n. 300, 00166 Roma, Italy. mecoma@rdn.it |
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Abstract: | In this paper an ultrasound assisted procedure for the extraction of the bio-available fraction of humic substance in marine sediments is described. The proposed method is based on a preliminary 24 h 8 M HCl treatment already proposed in a previous paper [M. Mecozzi, E. Pietrantonio, M. Amici, Fres. Environ. Bull. 7 (1998) 605], followed by consecutive extractions by 0.5 M NaOH coupling to an ultrasonic treatment. The main advantages of the ultrasound procedure are the reduced times of extraction which take 30 min in contrast to the 24 h required by shaking method and the possibility to perform also the quantitative estimation of the extractable fraction of humic substance present in marine samples. |
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