| Study on interaction between Dy（III）（NR）3 complex and herring sperm DNA by spectroscopy |
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| 作者姓名： | 张艳 王兴明 费丹 赵娜 赵婷婷 朱海峰 丁立生 |
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| 作者单位： | [1]Department of Chemistry, Material Science and Engineering College,Southwest University of Science and Technology, Mianyang 621010, China [2]Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China |
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| 基金项目： | This work was supported by the National Natural Science Foundation of China under Grant No. 30572254. We are grateful to the apparatus support of the Ana- lytical and Testing Center of Southwest University of Science and Technology. |
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| 摘 要： | The action mode between Dy（III）（NR）3 and herring sperm DNA is studied by ultraviolet-visible （UV- vis） and fluorescence spectra as well as electrochemistry. Double-reciprocal method studies show that the binding stoichiometry between Dy（III）（NR）3 and DNA is 1 ： 1, the binding constants at different temperatures are 1.15 ×10^5 L/tool at 25 ℃ and 2.09 × 105 L/mol at 35 ℃, and the corresponding I thermodynamic parameters are /krHm = 2.48 × 10^4 J/tool, ArGm ： -2.34 × 104 J/mol, ArSm = 161.7 J/（mol. K）, ArSm is the driving force in this reaction. Combined with Scatchard method and melting method, the results suggest that the interaction mode between Dy（III）（NR）3 and herring sperm DNA is intercalation fashion and groove fashion.
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| 关 键 词： | 鱼精子DNA 鲱鱼精DNA 荧光光谱 星期 相互作用 紫外可见 化学计量学 热力学参数 |
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