| Tetra-substituted Amino Aluminum Phthalocyanine as a New Red-region Fluorescent Substrate for Horseradish Peroxidase Based Enzyme-linked Immunosorbent Assay |
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| 引用本文： | 杨黄浩,李东辉,陈小兰,曲会英,丁马太,许金钩. Tetra-substituted Amino Aluminum Phthalocyanine as a New Red-region Fluorescent Substrate for Horseradish Peroxidase Based Enzyme-linked Immunosorbent Assay[J]. 中国化学, 2002, 20(12): 1573-1578. DOI: 10.1002/cjoc.20020201220 |
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| 作者姓名： | 杨黄浩 李东辉 陈小兰 曲会英 丁马太 许金钩 |
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| 作者单位： | YANG，Huang-Hao a，b LI，Dong-Hui c CHEN，Xiao-Lan a QU，Hui-Ying a DING，Ma-Tai b XU，Jin-Gou，a a Key Laboratory of Analytical Science of Ministry of Education，Department of Chemistry，Xiamen University，Xiamen，Fujian 361005，China b Department of Material Science，Xiamen University，Xiamen，Fujian 361005，China c Cancer Research Center，Xiamen University，Xiamen，Fujian 361005，China |
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| 基金项目： | theNationalNaturalScienceFoundationofChina (No.2 9775 0 21)andtheResearchFundfortheDoctoralProgramofHigherEducation |
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| 摘 要： | ＩｎｔｒｏｄｕｃｔｉｏｎＥｎｚｙｍｅｉｍｍｕｎｏａｓｓａｙｂａｓｅｄｏｎａｓｅｌｅｃｔｉｖｅａｎｔｉｇｅｎ ａｎｔｉｂｏｄｙｂｉｎｄｉｎｇａｎｄａｌａｂｅｌｅｎｚｙｍｅｈａｓｇａｉｎｅｄｉｎｃｒｅａｓ ｉｎｇｉｍｐｏｒｔａｎｃｅｉｎｒｅｃｅｎｔｙｅａｒｓ .Ａｍｏｎｇｔｈｅｅｎｚｙｍｅｓｕｓｅｄ ,ｈｏｒｓｅｒａｄｉｓｈｐｅｒｏｘｉｄａｓｅ (ＨＲＰ)ｉｓｔｈｅｍｏｓｔｗｉｄｅｌｙｕｓｅｄｅｎｚｙｍｅｌａｂｅｌｂｅｃａｕｓｅｏｆｉｔｓｈｉｇｈｓｐｅｃｉｆｉｃｉｔｙａｎｄｓｅｎｓｉｔｉｖｉ ｔｙ .1 3 Ｆｌｕ…
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Tetra-substituted Amino Aluminum Phthalocyanine as a New Red-region Fluorescent Substrate for Horseradish Peroxidase Based Enzyme-linked Immunosorbent Assay |
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| YANG,Huang-Hao a,b LI,Dong-Hui c CHEN,Xiao-Lan a QU,Hui-Ying a DING,Ma-Tai b XU,Jin-Gou,a a Key Laboratory of Analytical Science of Ministry of Education. Tetra-substituted Amino Aluminum Phthalocyanine as a New Red-region Fluorescent Substrate for Horseradish Peroxidase Based Enzyme-linked Immunosorbent Assay[J]. Chinese Journal of Chemistry, 2002, 20(12): 1573-1578. DOI: 10.1002/cjoc.20020201220 |
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| Authors: | YANG Huang-Hao a b LI Dong-Hui c CHEN Xiao-Lan a QU Hui-Ying a DING Ma-Tai b XU Jin-Gou a a Key Laboratory of Analytical Science of Ministry of Education |
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| Affiliation: | YANG,Huang-Hao a,b LI,Dong-Hui c CHEN,Xiao-Lan a QU,Hui-Ying a DING,Ma-Tai b XU,Jin-Gou,a a Key Laboratory of Analytical Science of Ministry of Education,Department of Chemistry,Xiamen University,Xiamen,Fujian 361005,China b Department of Material Science,Xiamen University,Xiamen,Fujian 361005,China c Cancer Research Center,Xiamen University,Xiamen,Fujian 361005,China |
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| Abstract: | The use of tetra‐substituted ammo aluminum phthalocyanine (TAAIPc) as a new red‐region fluorescent substrate for horseradish peroxidase (HRP)‐based enzyme‐linked immunosorbent assay was investigated. TAAIPc displayed an excitation maximum at 610 nm and emission maximum at 678 nm in a strong acidic medium. In the presence of HRP, trace amounts of H2O2 could rapidly and significantly react with TAAIPc, thus quenching the fluorescence of TAAIPc. The Michaelis‐Menten parameters Km and Vmax were measured to be 2.82 × 10?6 mol/L?1 and 6.0 × 10?9 mol·L?1, respectively. In this paper, TAAIPc was used in an HRP‐based enzyme‐linked immunosorbent assay (ELISA) of α‐fetoprotein (AFP) in human serum with satisfactory results. AFP could be determined in the concentration range of 0.5–200 ng/mL with a detection limit of 0.2 ng/mL, which was close to that of radioimmunoassay. The advantage of proposed method was strongly minimizing the interference resulting from background fluorescence or scattering light and had a high analytical sensitivity. |
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| Keywords: | tetra-substituted amino aluminum phthalocyanine red-region fluorescent substrate horseradish peroxidase enzyme-linked immunosorbent assay |
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