INACTIVATION OF PHAGE BY NEAR-ULTRAVIOLET RADIATION AND HYDROGEN PEROXIDE |
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| Authors: | A Eisenstark R L Buzard P S Hartman † |
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| Institution: | Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA |
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| Abstract: | A series of phage with different genomes (both single-stranded and double-stranded RNA and DNA) was inactivated with hydrogen peroxide (H2O2) in various combinations with far-ultraviolet (FUV) and near-ultraviolet (NUV) radiations. In every case but one (a lipid-coated phage), a sublethal H2O2 concentration greatly enhanced killing by NUV but not FUV. Moreover, this NUV/H2O2 synergism was oxygen independent and there was little if any host cell reactivation upon NUV plus H2O2 inactivation. These results suggest that these phage are inactivated by a common mechanism irrespective of nucleic acid composition, but that some phage genomes may be more vulnerable to NUV/H2O2 inactivation than others. |
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