高功率微波辐照对雄性大鼠生殖细胞的影响

 采用激光扫描共聚焦显微镜观察受不同参数高功率微波辐照后的大鼠精子细胞内Ca²⁺的变化，同时观察了辐照后大鼠的生殖细胞微核率、畸形率、精子数量、存活率和顶体酶反应的变化。实验表明：此条件下的高功率微波辐照对雄性大鼠的生殖细胞微核率和畸形率没有明显的损伤效应，但4×10⁵次脉冲的高功率微波辐照对大鼠的顶体酶反应有较明显的抑制作用，可能通过改变细胞内第二信使的Ca²⁺浓度来影响其生殖细胞功能。

High power microwave irradiation effects on sperm cells of male rats

The effect of HPM on the intracellular calcium of sperm cells was determined using laser scanning confocal microscopy. The abnormality,micronucleus frequency,livability, numbers and the acrosin reaction of the sperm cells were studied at the same time.The experiments indicate that there are no visible damaging effects on the abnormality and micronucleus frequency of the sperm cells after HPM irradiation,but the acrosin reaction of the rat sperm cells was restrained significantly after 4×10~5 pulses irradiation. The function of the sperm cells was probably induced by changing the intracytoplasmic calcium concentration of the rat sperm cells.