A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement |
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| Authors: | Xiao Yi Piorek Brian D Plaxco Kevin W Heeger Alan J |
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| Institution: | Department of Physics, Institute for Polymers and Organic Solids, Program in BioMolecular Science and Engineering, University of California, Santa Barbara, California 93106, USA. |
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| Abstract: | Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as approximately 3 nM. |
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