Enantiomeric Separation of Amino Alcohols by Ion-pair Chromatography

Enantiomers of four amino alcohols were resolved by ion-pair chromatography with (+)-10-camphorsulphonic acid as chiral counter ion. Studies of the influence of the mobile phase composition, the solute structure and the mobile phase flow-rate on separation are presented. Under the optimized conditions, enantiomeric propranolol, norephedrine, metropolol and salbutamol were separated using dichloromethane -1-pentanol (97:3, v/v) as mobile phase on Lichrospher-100-DIOL column.