| Cu(II), Fe(III)与人血清白蛋白相互作用的荧光光谱研究 |
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| 引用本文： | 梁宏,邢本刚,吴庆轩,罗济文,周永洽,申泮文.Cu(II), Fe(III)与人血清白蛋白相互作用的荧光光谱研究[J].化学学报,1999,57(2):161-165. |
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| 作者姓名： | 梁宏 邢本刚 吴庆轩 罗济文 周永洽 申泮文 |
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| 作者单位： | 广西师范大学化学系.桂林(541004);南开大学化学系.天津(300071) |
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| 基金项目： | 国家自然科学基金(29271025)，广西自然科学基金(青9453001) |
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| 摘 要： | 通过研究Cu(II),Fe(III)对人血清白蛋白(HSA)内源荧光的猝灭,探讨了Cu(II),Fe(III)与人血清白蛋白的结合机理。基于Forster非辐射能量转移机理。获得了人血清白蛋白第一类Cu(II)结合部位与214位色氨酸残基间的距离为1.8nm,并讨论了Cu(II),Fe(III)与HSA结合的差异。
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| 关 键 词： | 铜 铁 血清蛋白 荧光猝灭 清蛋白 荧光谱法 相互作用 |
| 修稿时间： | 1997年7月12日 |
Study on the interaction of human serum albumin with Cu(II) and Fe (III) by fluorescence method |
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| LIANG Hong,XING Ben-Gang,WU Qing-xuan,LUO Ji-Wen,ZHOU Yong-Qia,SHEN Pan-wen.Study on the interaction of human serum albumin with Cu(II) and Fe (III) by fluorescence method[J].Acta Chimica Sinica,1999,57(2):161-165. |
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| Authors: | LIANG Hong XING Ben-Gang WU Qing-xuan LUO Ji-Wen ZHOU Yong-Qia SHEN Pan-wen |
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| Institution: | Guangxi Normal Univ, Dept Chem.Guilin(541004);Nankai Univ, Dept Chem. Tianjin(300071) |
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| Abstract: | The mechanism of Cu(II) and Fe(III) binding to human serum albumin has been studied through the quenching of the intrinsic fluorescence of HSA. Based on Forster non-radiative energy transfer theory, the distance (1.8nm) between Trp-214 residue of HSA and the first class of binding site was determined. The different interaction of Cu(II), Fe(III) with HSA has also been discussed in this paper. |
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| Keywords: | quenching fluorescence human serum albumin binding sites non-radiative energy transfer |
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