基于EPR方法的天然产物抗氧化性能研究(英文)

自旋捕捉EPR、用于动物模型的低温EPR以及自旋标记EPR等多种EPR方法已经用于天然产物的抗氧化活性研究中.虽然对于抗氧化剂而言其必备条件之一就是具有体外清除自由基的能力,但是外源性抗氧化剂在生物体内抗氧化剂抗氧化能力评价的方法更有实际意义.通过对超过200种中药和一些传统中药处方的研究,已经建立并详细描述了用于评价天然产物抗氧化能力的EPR筛选方法.通过EPR方法可以获得天然产物在分子水平、细胞水平以及组织水平抗氧化能力的比较全面的评价.

Studies of Antioxidation Activity of Natural Products with EPR Methods

Many of EPR(electron paramagnetic resonance) experimental methods can be used in studies of antioxidation activities of natural products, such as spin trapping EPR, cryogenic EPR for modeled animals and spin labeling EPR et al. It is an elementary requirement for an antioxidant to scavenge oxygen free radicals in vitro, but evaluating method in vivo is more valuable when the antioxidant is applied to a biological system. Through the studies of more than 200 herbages and some TCM recipes, the EPR screening methods for natural products as antioxidants have been established and described in detail. With the above EPR methods, it is believed that antioxidation activities of natural products can be obtained at molecular, cell, and tissue levels, respectively.