Determination of a small amount of niacin in foodstuffs by high-performance liquid chromatography.

A high-performance liquid chromatographic method for the determination of a small amount of niacin in foodstuffs is described. This method is based on hydrolysis of sample, extraction, clean-up (anion-exchange and cation-exchange columns) and separation from interferences by chromatography on an amino phase column. The detection limit of niacin in foodstuff is about 0.01 mg per 100 g. The results of analysis of foodstuffs by this method are in good agreement with those obtained by the microbiological method.