| 催化荧光光度法测定血红蛋白 |
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| 引用本文： | 董存智,张淑静.催化荧光光度法测定血红蛋白[J].分析试验室,2010,29(1). |
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| 作者姓名： | 董存智 张淑静 |
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| 作者单位： | 淮北煤炭师范学院化学与材料科学学院,淮北,235000 |
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| 摘 要： | 基于血红蛋白(Hb)对过氧化氢氧化靛蓝胭脂红体系的催化作用,建立了模拟酶催化荧光光度法测定血红蛋白的新方法并对催化氧化反应的机理进行了初步探讨。实验发现体系的荧光强度与血红蛋白浓度在6.20×10-11~7.75×10-8mol/L范围内线性关系良好,方法的检出限为1.67×10-11mol/L。方法用于人血中血红蛋白含量的测定,回收率为98.8%~103.2%。
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| 关 键 词： | 血红蛋白 靛蓝胭脂红 催化荧光法 |
Catalytic fluorimetric determination of hemoglobin |
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| DONG Cun-zhi,ZHANG Shu-jing.Catalytic fluorimetric determination of hemoglobin[J].Chinese Journal of Analysis Laboratory,2010,29(1). |
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| Authors: | DONG Cun-zhi ZHANG Shu-jing |
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| Institution: | College of Chemistry and Material Science;Huaibei Coal Teachers College;Huaibei 235000 |
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| Abstract: | A new spectroflurometric method was developed for the determination of hemoglobin based on its catalytic activity of mimetic peroxidase on oxidation of indigo carmine in the presence of hydrogen peroxide. The mechanism of the catalytic reaction was studied briefly. The kinetic parameter of reaction and the optimum condition were discussed and the results indicated that hemoglobin concentration demonstrated good linear relationship in the range of 6.2×10~(-11)～7.75×10~(-8) mol/L with a detection limit of 1.67×10~(-11) mol/L. The method has been applied to determination of the hemoglobin concentration in human blood samples. The recoveries were in the range from 98.8% to 103.2%. |
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| Keywords: | Hemoglobin Indigo carmine Catalytic fluorimetry |
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