| 酶催化动力学光度法测定肾上腺素 |
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| 引用本文： | 陈亚红,田丰收. 酶催化动力学光度法测定肾上腺素[J]. 分析试验室, 2009, 28(8) |
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| 作者姓名： | 陈亚红 田丰收 |
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| 作者单位： | 周口师范学院化学系,周口,466000;周口师范学院化学系,周口,466000 |
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| 基金项目： | 河南省教育厅自然科学基金，河南省高校科技创新人才支持计划 |
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| 摘 要： | 基于肾上腺素对血红蛋白酶催化体系的抑制作用, 建立了酶催化动力学光度法测定肾上腺素的新方法. 实验研究了体系的最佳条件及动力学行为, 测定的线性范围为4.5×10-7～1.4×10-5 mol/L, 方法检出限为5.2×10-8 mol/L. 对浓度为9.0×10-6 mol/L的肾上腺素进行11次平行测定的相对标准偏差为3.5%. 此方法可用于药剂中肾上腺素含量的测定.
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| 关 键 词： | 肾上腺素 酶催化动力学光度法 血红蛋白 |
Determination of adrenaline by enzymatic catalytic spectrophotometry |
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| CHEN Ya-hong,TIAN Feng-shou. Determination of adrenaline by enzymatic catalytic spectrophotometry[J]. Chinese Journal of Analysis Laboratory, 2009, 28(8) |
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| Authors: | CHEN Ya-hong TIAN Feng-shou |
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| Affiliation: | Department of Chemistry;Zhoukou Normal University;Zhoukou 466000 |
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| Abstract: | A high sensitive and simple spectrophotometric method for the determination of adrenaline based on the inhibition of hemoglobin for the oxidation of H2O2 with acid chrome blue K was developed. The percentage inhibition (I%) of system is calculated under the optimal experimental conditions. The calibration curve is linear in the range of 4.5×10-7～1.4×10-5 mol/L with the detection limit of 5.2×10-8 mol/L. The relative standard deviation of this method is 3.5% for 11 determinations of 9.0×10-6 mol/L adrenaline. This method can be used for the determination of adrenaline in pharmaceuticals with satisfactory results. |
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| Keywords: | Adrenaline Enzymatic catalytic spectrophotometry Hemoglobin |
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