Direct determination of sialic acids in serum by capillary electrophoresis with UV detection |
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| Authors: | X L Dong Y Xu B C Lin |
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| Institution: | (1) Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, 457 Zhongshan Road, 116023 Dalian, P.R. China;(2) Dalian Third Municipal Hospital, 40 Qianshan Road, 116033 Dalian, P.R. China;(3) Dalian Obstetrics and Gynecology Hospital, 162 Zhongshan Road, 116012 Dalian, P.R. China |
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| Abstract: | Summary A novel method for the determination of N-acetylneuraminic acid (NANA) and N-glycolylneuraminic acid (NGNA) has been developed
using high-performance capillary electrophoresis with UV detection at 195 nm, without pre or post-column derivatisation. The
acids were separated in a 50-cm, fused-silica capillary (50μ i.d, 45.5-cm effective length) with Na2B4O7−Na2HPO4 buffer. The detection limit for NANA is a concentration of 9.6×10−6 M or, in terms of mass:3.879×10−14 mol (39 fmol). This method is applicable to determination of NANA in normal human serum. The results were also compared with
those of the colorimetrie method. |
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| Keywords: | Capillary electrophoresis Sialic acids Human serum |
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