Heterodyne interferometer for submicroscopic vibration measurements in the inner ear

Conditions in the inner ear for interferometric measurements are quite different from those encountered in other mechanical systems: (i) The inner ear is not mechanically stable, due to blood pulsations and breathing artifacts; (ii) access to the inner ear is limited by anatomical constraints that make it difficult to visualize the structures of interest; (iii) vibration amplitudes to be measured in the inner ear are very low; (iv) the structures in the inner ear are nearly transparent; therefore, the reflectivity is low and attempts to change this reflectivity artificially usually alter the response characteristics; (v) cells are subject to light damage if the incident light intensity is too high, which limits the laser power that can be utilized in the interferometer. A heterodyne interferometer specially designed to measure vibrations in the living inner ear is described. Theoretical and experimental characteristics of this instrument are discussed in detail. In contrast to the homodyne system, the measurement accuracy of this interferometer is not affected by the low-frequency animal movements. This system does not require attachment of a reference mirror to the animal, thereby providing an unobstructed view of the structure to be measured. It has a high linearity and dynamic range. Its vibration sensitivity is high (2.8 X 10(-13) m for 1-Hz bandwidth) even under the condition of low light reflectivity (0.02%), with 0.5-mW incident laser power.