| 胶束电动力学毛细管色谱法测定尿中核苷 |
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| 引用本文： | 郑育芳,张云,刘大渔,郭小亮,梅素容,熊建辉,孔宏伟,张朝,许国旺.胶束电动力学毛细管色谱法测定尿中核苷[J].高等学校化学学报,2001,22(6):912-915. |
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| 作者姓名： | 郑育芳 张云 刘大渔 郭小亮 梅素容 熊建辉 孔宏伟 张朝 许国旺 |
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| 作者单位： | 1. 中国科学院大连化学物理研究所国家色谱研究分析中心, 大连 116011;
2. 大连医科大学病理系, 大连 116011 |
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| 基金项目： | 国家自然科学基金! (批准号 :2 9775 0 2 4)，中国科学院院长特别基金!资助 |
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| 摘 要： | 用胶束电动力学毛细管色谱法测定尿中核苷.通过苯基硼酸亲和色谱柱对尿中核苷进行纯化,以十二烷基磺酸钠(SDS)-十水合四硼酸钠-磷酸二氢钠为缓冲液(pH=6.96),在未涂层石英毛细管上,于7kV恒压,29℃下进行电泳分离,检测波长为254nm.此方法用于28个正常人尿中14种核苷的测定,确定了正常成年人(汉族)尿中核苷的排放范围.
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| 关 键 词： | 胶束电动力学毛细管色谱法 核苷 尿 |
| 文章编号： | 0251-0790(2001)06-0912-04 |
| 收稿时间： | 2000-07-13 |
Analysis of Urinary Nucleosides by Micellar Electrokinetic Chromatography |
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| ZHENG Yu-Fang,ZHANG Yun,LIU Da-Yu,GUO Xiao-Liang,MEI Su-Rong,XIONG Jian-Hui,KONG Hong-Wei,ZHANG Chao,XU Guo-Wang.Analysis of Urinary Nucleosides by Micellar Electrokinetic Chromatography[J].Chemical Research In Chinese Universities,2001,22(6):912-915. |
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| Authors: | ZHENG Yu-Fang ZHANG Yun LIU Da-Yu GUO Xiao-Liang MEI Su-Rong XIONG Jian-Hui KONG Hong-Wei ZHANG Chao XU Guo-Wang |
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| Institution: | 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. Department of Pathology, Dalian Universityof Medicine, Dalian 116033, China |
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| Abstract: | Amicellar electrokinetic chromatography method for identification and quantitation of nucleosides in urine has been optimized. After nucleosides were extracted from human urine on phenyl boronic acid affinity chromatography column, the analysis was performed in an uncoated 52 cm×50 μm i.d. capillary tube using a 300 mmol/L SDS-25 mmol/Lborate-50 mmol/Lphosphate buffer (pH=6.96), 0.5 Psi×15 s injection, 7.0 kVvoltage and UVdetection at 254 nm. By using the MEKCmethod developed, 14 normal and modified nucleosides in 28 normal urines were quantified to establish the reference ranges. The result shows that this MEKCmethod is reliable, sensitive, efficient and suitable for clinical application. |
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| Keywords: | Micellar electrokinetic chromatography Nucleosides Urine |
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