微波消解样品-石墨炉原子吸收光谱法测定双黄连口服液及其药材中重金属元素

双黄连口服液及其中药材金银花、连翘和黄芩苷样品用硝酸-过氧化氢(3+1)混合溶液浸泡过夜,微波消解。并用石墨炉原子吸收光谱法(GF-AAS)测定了铅、镉、铬、铜、铁和锌的含量。磷酸二氢铵、8-羟基喹啉和硒(Ⅱ)分别被用作测定铅,镉,铜和铁、铬以及锌的基体改进剂。在优化的试验条件下,方法的回收率在90.4%～106.0%之间,相对标准偏差(n=5)在1.8%～4.6%之间。试验结果表明,双黄连口服液及其中药材中铁、锌、铜的含量较为丰富,铅和镉含量低于"药用植物及制剂外经贸绿色行业标准"。

GF-AAS Determination of Heavy Metal Elements in Shuanghuanglian Oral Liquor and Its Raw Medicines with Microwave Assisted Sample Digestion

Sample of Shuanghuanglian Oral Liquor and its raw medicines(i.e.Lonicera,Forsythia suspensa and Baicalin) were soaked overnight with mixture of HNO3 and H2O2(3+1),and then digested in the microwave digestor.The lead,cadmium,chromium,copper,iron and zinc in the sample solution were determined by GF-AAS.Ammonium dihydrogen phosphate was used as matrix modifier for the determination of Pb,Cd,Cu and Fe,8-hydroxyquinoline as matrix modifier for Cr,and selenium for Zn.Under optimized working conditions of the instrument,values of recovery and RSD′s(n=5) found were in the ranges of 90.4%-106.0% and 1.8%-4.6% respectively.As shown by the experimental results,the oral liquor was rich in Fe,Zn and Cu,while the contents of harmful elements Pb and Cd were found to be lower than that qualified in the "green trade standard".