a Biophysical Chemistry Laboratory, University of Sofia, James Bourchier Str., 1, 1126, Sofia, Bulgaria
b Laboratoire de Lipolyse Enzymatique, CNRS, 13402, Marseille Cedex 20, France
Abstract:
A detailed kinetic model describing the surface transformation of spread liposomes along with their enzymatic hydrolysis was developed. The model was applied to the hydrolysis of the long-chain phosphatidylcholine generating reaction products which remain at the interface and to medium-chain substrates from which the products desorb rapidly into the bulk phase. The overall kinetic constants of the hydrolysis in liposomal systems were compared with those obtained with monolayers under barostatic conditions. The values of the interfacial Michaelis-Menten constant were estimated.