Simultaneous determination of fat- and water-soluble vitamins by microemulsion electrokinetic chromatography |
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Authors: | E. P. Svidritskii E. B. Pashkova A. V. Pirogov O. A. Shpigun |
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Affiliation: | 1.Department of Chemistry,Moscow State University,Moscow,Russia |
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Abstract: | A new procedure was developed for the simultaneous determination of water- and fat-soluble vitamins by microemulsion electrokinetic chromatography. Using microemulsions based on sodium dodecyl sulfate, Brij 35, 1-butanol, and heptane, 10 vitamins were separated (4 water-soluble and 6 fat-soluble) within 35 min. The efficiency of the separation was 800000 theoretical plates (effective capillary length, 40 cm). To enhance the selectivity, 2-propanol was used as a hydrophobizing addition into the microemulsion. The procedure was used for analyzing a kaolin-based vitamin premix. The use of microemulsion as an agent for extracting vitamins from the support considerably simplifies the sample preparation. |
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