Effectiveness of Phosphate Groups in Noncovalent Interactions in Binary Adenosine Nucleotides/Phosphoserine Aqueous Systems |
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Authors: | Renata Jastrzab Lechoslaw Lomozik |
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Institution: | (1) Faculty of Chemistry, A. Mickiewicz University, 60-780 Poznan, Poland |
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Abstract: | Adduct formation in binary systems of O-phospho-L-serine (Ser-P) with adenosine 5′-monophosphate (AMP), adenosine 5′-diphosphate (ADP) and adenosine 5′-triphosphate (ATP), has been investigated. This study was performed in aqueous solutions using a potentiometric method with computer analysis
of the data, together with 13C and 31P NMR spectroscopic measurements. The overall stability constants of the adducts and the equilibrium constants for their formation
have been determined. Ion-dipole and ion-ion interactions have been established to occur in the identified noncovalent complexes.
An analysis of the equilibrium constants of the reaction has allowed the determination of the effectiveness of the phosphate
groups and donor atoms of heterocyclic rings for molecular complex formation. The potential reaction centers are the atoms
N(1) and N(7) from the purine base, the phosphate group of the nucleotides, and the phosphate, carboxyl and amine groups from
phosphorylated serine. Sites for the interactions in the bioligands have been found on the basis of an equilibrium constant
study and an analysis of the changes in the signal positions of their NMR spectra. |
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Keywords: | Phosphoserine Nucleotide Non-covalent interaction Potentiometric study NMR |
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