微流控芯片系统流式细胞术及单细胞荧光检测

微流控芯片系统用于细胞分析是近年来该技术发展的一个热点，因而受到越来越多的关注．其主要原因在于微全分析系统具备高度微型化、集成化和设计灵活等特点，通过巧妙设计和精密加工能够实现细胞的培养、凋亡及检测等功能．流式细胞术(Flow cytometer，FCM)是一种在功能水平上对单细胞或其它生物粒子进行定量分析和分选的检测手段，

Flow Cytometry and Single Cell Fluorescence Detection on a Microfluidic Device

A microfluidic device was developed for flow cytometry and single cell fluorescence detection. TO-PRO-3 as a vital DNA dye was used to label dead cells and some of apoptosis cells which could be penetrated from their broken membranes and intercalated into DNA chains. Cells were driven by combined effect of hydrodynamic and electric power and passed through the detection point one by one in micro channels. A laser induced fluorescence detection system which employed a 635 nm semiconductor laser was the only measurement of the whole system. This micro flow cytometor system was applied to estimate the damage degree of those HeLa cells having been exposed to ultraviolet radiation for 10, 20 and 40 min, respectively by the number of cells determined and their fluorescence intensity.