Organized Aggregation of Porphyrins in Lipid Bilayers for Third Harmonic Generation Microscopy |
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| Authors: | Dr Liyang Cui Dr Danielle Tokarz Dr Richard Cisek Dr Kenneth K Ng Prof Fan Wang Dr Juan Chen Prof Virginijus Barzda Prof Gang Zheng |
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| Institution: | 1. Princess Margaret Cancer Center and Techna Institute, UHN (Canada) http://www.utoronto.ca/zhenglab;2. Medical Isotopes Research Center, Peking University (China);3. Department of Medical Biophysics, University of Toronto (Canada);4. Department of Chemical and Physical Sciences and Department of Physics, University of Toronto (Canada) |
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| Abstract: | Nonlinear optical microscopy has become a powerful tool for high‐resolution imaging of cellular and subcellular composition, morphology, and interactions because of its high spatial resolution, deep penetration, and low photo‐damage to tissue. Developing specific harmonic probes is essential for exploiting nonlinear microscopic imaging for biomedical applications. We report an organized aggregate of porphyrins (OAP) that formed within lipidic nanoparticles showing fingerprint spectroscopic properties, structure‐associated second harmonic generation, and superradiant third harmonic generation. The OAP facilitated harmonic microscopic imaging of living cells with significantly enhanced contrast. The structure‐dependent switch between harmonic (OAP‐intact) and fluorescence (OAP‐disrupted) generation enabled real‐time multi‐modality imaging of the cellular fate of nanoparticles. Robustly produced under various conditions and easily incorporated into pre‐formed lipid nanovesicles, OAP provides a biocompatible nanoplatform for harmonic imaging. |
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| Keywords: | harmonic generation multimodality imaging nonlinear optical properties organized aggregation porphyrin nanoparticles |
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