Quantitation of free sulfate and total sulfoesters in human breast milk by ion chromatography |
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Authors: | M D McPhee S A Atkinson D E Cole |
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Institution: | I.W.K. Children's Hospital, Halifax, Nova Scotia, Canada. |
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Abstract: | A method for the assay of free sulfate and total sulfoesters in human breast milk by ion chromatography is described. After incubation in 1 M hydrochloric acid at 95 degrees C for 90 min, hydrolytic cleavage of sulfoester standards was essentially complete. The increase in free sulfate after hydrolysis was used as a measure of total acid-labile sulfoesters. We found that this fraction 222 +/- 16 mumol/l (mean +/- standard error), n = 29] comprised 87% of the total sulfate in mature milk. Free sulfate (35 +/- 3 mumol/l) therefore makes only a small contribution to the total sulfate pool available to human infants. |
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