高效液相色谱法测定化妆品中10种限用物质

建立了乙腈超声提取、高效液相色谱/二极管阵列检测快速测定化妆品中的氢醌、水杨酸、苯酚、苯氧乙醇、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯、双氯酚、三氯生10种限用物质含量的分析方法.对提取方法、液相色谱条件和检测条件进行了优化,10种限用物质标准溶液的线性范围为0.01～0.1g/L,50...

Determination of 10 Kinds of Restricted Substances in Cosmetics by High Performance Liquid Chromatography with Photodiode Array Detector

A high performance liquid chromatography(HPLC) coupled with photodiode array detector(PDAD) method was developed for the determination of content of hydroquinone,phenol,salicylic acid,2-phenoxyethanol,methyl 4-hydroxybenzoate,ethyl 4-hydroxybenzoate,propyl 4-hydroxybenzoate,butyl 4-hydroxybenzoate,dichlorophen and triclosan in cosmetics sample.The restricted substances were extracted with acetonitrile in sonic bathing for 10 min.The chromatographic separation was completed with a column of Lichrosorp RP-18(250 mm×4.6 mm,5 μm) using methanol-acetonitrile-ammonium acetate(pH 4.0) as mobile phase.The quantification was carried out by the external standard method.The calibration curves of 10 restricted substances were linear in the range of 0.01-0.1 g/L.The recoveries at three spiked levels of 50,100,200 mg/kg ranged from 80% to 113%.The relative standard deviations(RSD,n=7) ranged from 2.2% to 10.0% and the quantitation limits were in the range of 10-50 mg/kg.The method was rapid,easy,accurate and sensitive,and could meet the detection requirements for domestic and foreign limited substances in cosmetics.