1,3,5,7-四甲基-8-丙酰肼-二氟化硼-二吡咯甲烷柱前衍生高效液相色谱测定白酒中的脂肪醛

以合成的1,3,5,7-四甲基-8-丙酰肼-二氟化硼-二吡咯甲烷(BODIPY-丙酰肼)作为柱前荧光衍生试剂,用于高效液相色谱分离荧光法检测甲醛、乙醛、丙醛、丁醛、戊醛、己醛、庚醛、辛醛、壬醛、癸醛、十一醛、十二醛等12种脂肪醛.通过对衍生条件和分离条件进行优化,建立了测定脂肪醛的HPLC/FD新方法.衍生反应以H3P...

Determination of Aliphatic Aldehydes in Wine by High Performance Liquid Chromatography with BODIPY based Hydrazine Reagent

A new fluorescent labeling reagent,1,3,5,7-tetramethyl-8-propionohydrazide-difluoroboradiaza-s-indacene(BODIPY-propionohydrazide),was synthesized for the determination of C1-C12 aliphatic aldehydes by high performance liquid chromatography(HPLC).The experimental conditions were optimized.In the presence of phosphorous acid as a catalyst,the derivatization reaction of BODIPY-propionohydrazide with aldehydes was completed in 30 min at 60 ℃.The stable BODIPY hydrazone derivatives(except for formaldehyde and acetaldehyde derivatives) could be separated in 20 min with acetonitrile(containing 20% tetrahydrofuran) and HCOOH-NH3·H2O as mobile phase(pH=6.5).Under the optimal conditions,the calibration curves of C1-C12 aliphatic aldehydes were linear in the range of 0.01-2.00 μmol·L-1 with detection limits(S/N=3) of 0.23-0.78 nmol·L-1.The established method was applied in the determination of aliphatic aldehydes in wine samples with spiked recoveries of 95%-105%.