| 高效液相色谱分离-在线光化学衍生/荧光光谱法测定5种水溶性维生素的研究 |
| |
| 引用本文： | 刘珺,方芳,陈婷,段华玲,弓振斌.高效液相色谱分离-在线光化学衍生/荧光光谱法测定5种水溶性维生素的研究[J].分析测试学报,2011,30(10):1100-1106. |
| |
| 作者姓名： | 刘珺 方芳 陈婷 段华玲 弓振斌 |
| |
| 作者单位： | 1. 厦门大学,近海海洋环境科学国家重点实验室,福建,厦门,361005;厦门大学海洋与环境学院,福建,厦门,361005
2. 厦门大学海洋与环境学院,福建,厦门,361005 |
| |
| 基金项目： | University research grant from Agilent Technologies Foundation,USA |
| |
| 摘 要： | 建立了高效液相色谱分离-在线光化学衍生/荧光光谱法测定水溶性维生素烟酸(NIA)、烟酸胺(NIC)、B1、B12及B2的新方法.以含有0.018 mol/L三乙胺、0.002 mol/L庚烷磺酸钠的0.05 mol/L磷酸盐缓冲液(A相,pH 5.8)和甲醇(B相)为流动相(85:15),等度洗脱分离5种水溶性维生素;...
|
| 关 键 词： | 水溶性维生素 光化学衍生 荧光检测 高效液相色谱 |
Simultaneous Determination of 5 Water-soluble Vitamins by HPLC with On-line Photochemical Derivatization and Fluorescence Detection |
| |
| LIU Jun,FANG Fang,CHEN Ting,DUAN Hua-ling,GONG Zhen-bin.Simultaneous Determination of 5 Water-soluble Vitamins by HPLC with On-line Photochemical Derivatization and Fluorescence Detection[J].Journal of Instrumental Analysis,2011,30(10):1100-1106. |
| |
| Authors: | LIU Jun FANG Fang CHEN Ting DUAN Hua-ling GONG Zhen-bin |
| |
| Institution: | LIU Jun1,2,FANG Fang2,CHEN Ting2,DUAN Hua-ling1,GONG Zhen-bin1,2 (1.State Key Laboratory of Marine Environmental Science,Xiamen University,Xiamen 361005,China,2.College of Oceanography & Environmental Science,China) |
| |
| Abstract: | A high performance liquid chromatographic(HPLC) method using on-line photochemical derivatization and fluorescence detection was developed for the simultaneous determination of nicotinic acid(NIA),nicotinamide(NIC),thiamine hydrochloride(B1),riboflavin(B2) and cyanocobalamin(B12).A self-built time/energy programmed photochemical reactor(PCR) with an ultraviolet mercury lamp was installed between the diode-array detector(DAD) and fluorescence detector(FLD) to convert the non-or weakly fluorescent vitamins in... |
| |
| Keywords: | water-soluble vitamins photochemical derivatization fluorescence detection high performance liquid chromatography |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《分析测试学报》浏览原始摘要信息 |
| 点击此处可从《分析测试学报》下载免费的PDF全文 |
|
