多壁碳纳米管净化/超高效液相色谱串联质谱同时测定动物组织中四环素与喹诺酮多残留

采用超高效液相色谱-串联质谱(UPLC-MS/MS)在正离子模式下通过多反应监测(MRM)方式同时测定了猪肉和鸡肉中3种四环素和2种喹诺酮类药物的残留量.残留药物经Mcllvaine-Na2EDTA缓冲溶液(pH4.0)提取后,采用多壁碳纳米管(WMCNTs)固相萃取柱净化,甲酸-乙腈(体积比5:95)洗脱,UPLC-...

Simultaneous Determination of Tetracyclines and Quinolones Residues in Animal Tissue by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry and Multiwalled Carbon Nanotubes Cleaning

An analytical method was developed and validated for the simultaneous determination of 3 tetracyclines and 2 quinolones in pork and chicken by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) by positive ion mode under multiple reaction monitoring(MRM).The sample were extracted by ultrasonic extraction in Mcllvaine-Na2EDTA buffer(pH 4.0).The extract was cleaned up on a solid phase extraction(SPE) cartridge packed with multiwalled carbon nanotubes(MWCNTs) and using formic acid-acetonitrile(5 ∶95,by volume) mixture as eluting solvent.The drugs residues were analyzed by UPLC-MS/MS.The analysis time was only 10 min.Good linearities were obtained in the range of 5-500 μg/L.The limit of detection(LOD) was 15 μg/L for chlortetracycline(CTC) and 5 μg/L for the other four drugs.The recoveries at spiked levels of 50,100,200 μg/L ranged from 76% to 90% for 5 drugs with relative standard deviations(RSDs) less than 9%.With good stability and high sensitivity,the method could be used for the rapid determinaion of these drug residues in pork and chicken sample.